Naoxintong capsule (NXTC) was a famous patent medicine of Traditional Chinese Medicine (TCM) to treat cerebrovascular\ndiseases in China. An LC-MS/MS method was developed for simultaneous determination of 11 major ingredients (paeoniflorin,\necdysterone, amygdalin, mulberroside A, caffeic acid, ferulic acid, salvianolic acid B, astragaloside IV, formononetin, cryptotanshinone,\nand tanshinone IIA) in NXTC in rat plasma. All analytes were separated on an Eclipse plus C18 column using a gradient\nmobile phase system of acetonitrile-0.1% formic acid aqueous solution. The lower limits of quantification of 11 ingredients were\nbetween 0.075 and 10 ngmLâË?â??1.The precision was less than 15% and the accuracies were between 85% and 115%. The results showed\nthat caffeic acid, ferulic acid, formononetin, cryptotanshinone, and tanshinone IIA could be detected after oral administration of\nNXTC. The validated method was successfully applied to pharmacokinetic study of the caffeic acid, ferulic acid, formononetin,\ncryptotanshinone, and tanshinone IIA in rats after oral administration of NXTC at single and triple dose.
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